Product Info Summary
SKU: | A05668-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Alpha Taxilin/TXLNA Antibody Picoband™
View all alpha-Taxilin Antibodies
SKU/Catalog Number
A05668-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha Taxilin/TXLNA Antibody Picoband™ catalog # A05668-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Alpha Taxilin/TXLNA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05668-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Alpha Taxilin/TXLNA recombinant protein (Position: M1-A546).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A05668-1 is reactive to Txlna in Human
Applications
A05668-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
101272 MW
Background of alpha-Taxilin
Alpha-taxilin also known as interleukin-14 (IL-14) or high molecular weight B-cell growth factor (HMW-BCGF) is a protein that in humans is encoded by the TXLNA gene. Predicted to enable syntaxin binding activity. Predicted to be involved in exocytosis. Predicted to act upstream of or within B cell activation. Located in cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a05668 1 txlna primary antibodies wb testing 1 a05668 1 txlna primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05668-1-txlna-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Alpha Taxilin/TXLNA using anti-Alpha Taxilin/TXLNA antibody (A05668-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Taxilin/TXLNA antigen affinity purified polyclonal antibody (Catalog # A05668-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Taxilin/TXLNA at approximately 75 kDa. The expected band size for Alpha Taxilin/TXLNA is at 62 kDa.
![a05668 1 txlna primary antibodies if testing 2 a05668 1 txlna primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a05668-1-txlna-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of Alpha Taxilin/TXLNA using anti-Alpha Taxilin/TXLNA antibody (A05668-1).
Alpha Taxilin/TXLNA was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Alpha Taxilin/TXLNA Antibody (A05668-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a05668 1 txlna primary antibodies fcm testing 3 a05668 1 txlna primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a05668-1-txlna-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of U251 cells using anti-Alpha Taxilin/TXLNA antibody (A05668-1).
Overlay histogram showing U251 cells stained with A05668-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Taxilin/TXLNA Antibody (A05668-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a05668 1 txlna primary antibodies fcm testing 4 a05668 1 txlna primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a05668-1-txlna-primary-antibodies-fcm-testing-4.png)
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Figure 4. Flow Cytometry analysis of U87 cells using anti-Alpha Taxilin/TXLNA antibody (A05668-1).
Overlay histogram showing U87 cells stained with A05668-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Taxilin/TXLNA Antibody (A05668-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Txlna (Source: Uniprot.org, NCBI)
Gene Name
Txlna
Full Name
Alpha-taxilin
Weight
101272 MW
Superfamily
taxilin family
Alternative Names
alphaTaxilin; alpha-Taxilin; DKFZp451J0118; IL14; IL-14; interleukin 14; MGC118870; MGC118871; RP4-622L5.4; taxilin alpha; TXLN; TXLNA Txlna|2600010N21Rik, AI425952, AW209241, IL, Il14, Tx, Txln|taxilin alpha|alpha-taxilin|interleukin 14|taxilin Txln
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Txlna, check out the Txlna Infographic
![Txlna infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Txlna: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha Taxilin/TXLNA Antibody Picoband™ (A05668-1)
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