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Product Info Summary
| SKU: | P00215 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Alpha Synuclein (Ser129) SNCA Antibody
View all alpha-Synuclein Antibodies
SKU/Catalog Number
P00215
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Alpha Synuclein (Ser129) SNCA Antibody (Catalog # P00215). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Alpha Synuclein (Ser129) SNCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00215)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 of rat alpha-aynuclein, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00215 is reactive to SNCA in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
14460 MW
Background of alpha-Synuclein
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alphasynuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at Ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00215 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC: 1:200
Validation Images & Assay Conditions
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Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For SNCA (Source: Uniprot.org, NCBI)
Gene Name
SNCA
Full Name
Alpha-synuclein
Weight
14460 MW
Superfamily
synuclein family
Alternative Names
Alpha synuclein antibody, Alpha-synuclein antibody, Alpha-synuclein, isoform NACP140 antibody, alphaSYN antibody, MGC105443 antibody, MGC110988 antibody, MGC127560 antibody, MGC64356 antibody, NACP antibody, Non A beta component of AD amyloid antibody, Non A4 component of amyloid antibody, Non A4 component of amyloid precursor antibody, Non-A beta component of AD amyloid antibody, Non-A-beta component of alzheimers disease amyloid , precursor of antibody, Non-A4 component of amyloid precursor antibody, Non-A4 component of amyloid, precursor of antibody, OTTHUMP00000218549 antibody, OTTHUMP00000218551 antibody, OTTHUMP00000218552 antibody, OTTHUMP00000218553 antibody, OTTHUMP00000218554 antibody, PARK 1 antibody, PARK 4 antibody, PARK1 antibody, PARK4 antibody, Parkinson disease (autosomal dominant Lewy body) 4 antibody, Parkinson disease familial 1 antibody, SNCA antibody, Snca synuclein alpha (non A4 component of amyloid precursor) antibody, SYN antibody, Synuclein alpha antibody, Synuclein alpha 140 antibody, Synuclein alpha (non A4 component of amyloid precursor) antibody, SYUA_HUMAN antibody SNCA NACP, PARK1, PARK4, PD1 synuclein alpha alpha-synuclein|I+/--synuclein|non A-beta component of AD amyloid|synuclein alpha-140|synuclein, alpha (non A4 component of amyloid precursor)|truncated alpha synuclein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNCA, check out the SNCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Alpha Synuclein (Ser129) SNCA Antibody (P00215)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-Alpha Synuclein (Ser129) SNCA Antibody
Question
Is there a BSA free version of anti-Phospho-Alpha Synuclein (Ser129) antibody P00215 available?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Alpha Synuclein (Ser129) antibody P00215 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-Phospho-Alpha Synuclein (Ser129) antibody P00215. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
Would P00215 anti-Phospho-Alpha Synuclein (Ser129) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Carter
Verified customer
Asked: 2019-11-07
Answer
You can see on the product datasheet, P00215 anti-Phospho-Alpha Synuclein (Ser129) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
