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Product Info Summary
| SKU: | A05022-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Rabbit |
| Host: | Goat |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Aldolase ALDOA Antibody
View all Aldolase A Antibodies
SKU/Catalog Number
A05022-1
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Aldolase ALDOA Antibody (Catalog # A05022-1). Tested in ELISA, IP, WB applications. This antibody reacts with Human, Rabbit.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Aldolase ALDOA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05022-1)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Aldolase [Rabbit Muscle]
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05022-1 is reactive to ALDOA in Human, Rabbit
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
27751 MW
Background of Aldolase A
Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05022-1 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:7,000
IP: 1:100
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
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Anti aldolase antibody
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Figure 1. Western blot analysis of ALDOA using anti-ALDOA antibody (A05022-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for ALDOA.
Click image to see more details
Figure 2. Western blot analysis of ALDOA using anti-ALDOA antibody (A05022-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for ALDOA.
Click image to see more details
Figure 3. Western blot analysis of ALDOA using anti-ALDOA antibody (A05022-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for ALDOA.
Protein Target Info & Infographic
Gene/Protein Information For ALDOA (Source: Uniprot.org, NCBI)
Gene Name
ALDOA
Full Name
Fructose-bisphosphate aldolase A
Weight
27751 MW
Superfamily
class I fructose-bisphosphate aldolase family
Alternative Names
Aldolase, Fructose-bisphosphate aldolase A, Muscle-type aldolase ALDOA ALDA, GSD12, HEL-S-87p aldolase, fructose-bisphosphate A fructose-bisphosphate aldolase A|aldolase A, fructose-bisphosphate|epididymis secretory sperm binding protein Li 87p|fructose-1,6-bisphosphate triosephosphate-lyase|lung cancer NY-LU-1|muscle-type aldolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDOA, check out the ALDOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aldolase ALDOA Antibody (A05022-1)
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Customer Q&As
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11 Customer Q&As for Anti-Aldolase ALDOA Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Aldolase antibody A05022-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Is there a BSA free version of anti-Aldolase antibody A05022-1 available?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Aldolase antibody A05022-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-19
Question
Will anti-Aldolase antibody A05022-1 work on primate WB with testis uterus?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Our lab technicians have not validated anti-Aldolase antibody A05022-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Aldolase antibody A05022-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate testis uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-13
Question
Does A05022-1 anti-Aldolase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
As indicated on the product datasheet, A05022-1 anti-Aldolase antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-Aldolase antibody for IP for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
As indicated on the product datasheet, A05022-1 anti-Aldolase antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-Aldolase antibody A05022-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-11
Question
Please see the WB image, lot number and protocol we used for eye using anti-Aldolase antibody A05022-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
Would anti-Aldolase antibody A05022-1 work for IP with eye?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
According to the expression profile of eye, ALDOA is highly expressed in eye. So, it is likely that anti-Aldolase antibody A05022-1 will work for IP with eye.
Boster Scientific Support
Answered: 2018-08-02
Question
My question regarding product A05022-1, anti-Aldolase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Bhatt
Verified customer
Asked: 2017-07-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05022-1 anti-Aldolase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-11
Question
Is a blocking peptide available for product anti-Aldolase antibody (A05022-1)?
C. Miller
Verified customer
Asked: 2015-04-23
Answer
We do provide the blocking peptide for product anti-Aldolase antibody (A05022-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-23
Question
We need to test anti-Aldolase antibody A05022-1 on human eye for research purposes, then I may be interested in using anti-Aldolase antibody A05022-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Taylor
Verified customer
Asked: 2014-09-18
Answer
The products we sell, including anti-Aldolase antibody A05022-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-09-18
