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Product Info Summary
| SKU: | M08868 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-ALDH6A1 Antibody
SKU/Catalog Number
M08868
Size
80 µl
Description
Boster Bio Anti-ALDH6A1 Antibody (Catalog # M08868). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ALDH6A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08868)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
147CT8.3.4
Isotype
IgG1,IgK
Immunogen
This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08868 is reactive to ALDH6A1 in Human
Reconstitution
Calculated molecular weight
57840 Da
Background of ALDH6A1
This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08868 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. M08868 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
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Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For ALDH6A1 (Source: Uniprot.org, NCBI)
Gene Name
ALDH6A1
Full Name
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
Weight
57840 Da
Superfamily
aldehyde dehydrogenase family
Alternative Names
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH ALDH6A1 MMSADHA, MMSDH aldehyde dehydrogenase 6 family member A1 methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial|malonate-semialdehyde dehydrogenase (acetylating)|mitochondrial acylating methylmalonate-semialdehyde dehydrogenase|testicular tissue protein Li 122
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDH6A1, check out the ALDH6A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDH6A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALDH6A1 Antibody (M08868)
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