Product Info Summary
SKU: | M00725-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Rat |
Host: | Mouse |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Akt2 Monoclonal Antibody
SKU/Catalog Number
M00725-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Akt2 Monoclonal Antibody catalog # M00725-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Akt2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00725-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1B6
Isotype
IgG
Immunogen
Purified recombinant fragment of human Akt2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00725-2 is reactive to AKT2 in Human, Monkey, Rat
Reconstitution
Calculated molecular weight
55769 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00725-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Immunofluorescent staining data of AKT2 using Anti-Akt2 Monoclonal Antibody (M00725-2).
Immunofluorescence (IF) analysis of PANC-1 (left) and HeLa (right) cells using Akt2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of AKT2 using Anti-Akt2 Monoclonal Antibody (M00725-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded human Thymus tissues with AEC staining using Akt2 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Akt2 Monoclonal Antibody M00725-2
Western Blot (WB) analysis using Akt2 Monoclonal Antibody against A431 (1)Protein Target Info & Infographic
Gene/Protein Information For AKT2 (Source: Uniprot.org, NCBI)
Gene Name
AKT2
Full Name
RAC-beta serine/threonine-protein kinase
Weight
55769 MW
Superfamily
protein kinase superfamily
Alternative Names
RAC-beta serine/threonine-protein kinase;2.7.11.1;Protein kinase Akt-2;Protein kinase B beta;PKB beta;RAC-PK-beta;AKT2; AKT2 HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA AKT serine/threonine kinase 2 RAC-beta serine/threonine-protein kinase|PKB beta|RAC-PK-beta|murine thymoma viral (v-akt) homolog-2|protein kinase Akt-2|protein kinase B beta|putative v-akt murine thymoma viral oncoprotein 2|rac protein kinase beta|v-akt murine thymoma viral oncogene homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT2, check out the AKT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Akt2 Monoclonal Antibody (M00725-2)
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