Product Info Summary
SKU: | M15791 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-AKR7L Antibody (N-Term)
SKU/Catalog Number
M15791
Size
80 µl
Description
Boster Bio Anti-AKR7L Antibody (N-Term) (Catalog # M15791). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AKR7L Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M15791)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AKR7L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-64 amino acids from human AKR7L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M15791 is reactive to AKR7L in Human
Reconstitution
Calculated molecular weight
36970 Da
Background of AKR7L
Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M15791 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling AKR7L with M15791 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Click image to see more details
Anti-AKR7L Antibody (N-Term)at 1:2000 dilution + HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For AKR7L (Source: Uniprot.org, NCBI)
Gene Name
AKR7L
Full Name
Aflatoxin B1 aldehyde reductase member 4
Weight
36970 Da
Superfamily
aldo/keto reductase family
Alternative Names
Aflatoxin B1 aldehyde reductase member 4, 1---, AFB1 aldehyde reductase 3, AFB1-AR 3, Aldoketoreductase 7-like, AKR7L, AFAR3, AKR7A4 AKR7L AFAR3, AFB1-AR 3, AFB1-AR3, AKR7A4 aldo-keto reductase family 7 like (gene/pseudogene) aflatoxin B1 aldehyde reductase member 4|AFB1 aldehyde reductase 3|aldo-keto reductase family 7 pseudogene|aldo-keto reductase family 7-like|aldoketoreductase 7-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKR7L, check out the AKR7L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKR7L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKR7L Antibody (N-Term) (M15791)
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