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Product Info Summary
| SKU: | M05378 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IP, IHC-F, ICC, WB |
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Product info
Product Name
Anti-AHNAK1 Purified Monoclonal Antibody
SKU/Catalog Number
M05378
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AHNAK1 Purified Monoclonal Antibody (Catalog# M05378). Tested in WB, IHC-F, ICC, IP application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-AHNAK1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05378)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-09
Isotype
Mouse IgG1
Immunogen
Bacterially expressed fragment of N-terminal domain of human AHNAK1. The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M05378 is reactive to AHNAK1 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
629101 MW
Background of AHNAK
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05378 is guaranteed for IP, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).
Immunocytochemistry staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Figure 2. Immunocytochemistry staining of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).
Immunocytochemistry staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).
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Figure 3. Immunohistochemistry validation of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).
Immunohistochemistry staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green)Protein Target Info & Infographic
Gene/Protein Information For AHNAK1 (Source: Uniprot.org, NCBI)
Gene Name
AHNAK1
Full Name
Neuroblast differentiation-associated protein AHNAK
Weight
629101 MW
Alternative Names
Desmoyokin AHNAK AHNAKRS, PM227 AHNAK nucleoprotein neuroblast differentiation-associated protein AHNAK|AHNAK-related|desmoyokin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AHNAK1, check out the AHNAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AHNAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AHNAK1 Purified Monoclonal Antibody (M05378)
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