Product Info Summary
SKU: | RP1071 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-AGFG1 Antibody
SKU/Catalog Number
RP1071
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AGFG1 Antibody catalog # RP1071. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AGFG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1071)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1071 is reactive to AGFG1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
58260 MW
Background of AGFG1
Arf-GAP domain and FG repeats-containing protein 1 is aprotein that in humans is encoded by the AGFG1 gene. AGFG1 is located to 2q36.3. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1071 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Testis Tissue, Rat Liver Tissue, Human Placenta Tissue, MCF-7 Whole Cell, HELA Whole Cell
ICC/IF: U20S cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Anti-AGFG1 antibody, RP1071, Western blotting
All lanes: Anti AGFG1 (RP1071) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
Click image to see more details
Figure 2. IF analysis of AGFG1 using anti-AGFG1 antibody (RP1071).
AGFG1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AGFG1 Antibody (RP1071) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of SiHa cells using anti-AGFG1 antibody (RP1071).
Overlay histogram showing SiHa cells stained with RP1071 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGFG1 Antibody (RP1071,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For AGFG1 (Source: Uniprot.org, NCBI)
Gene Name
AGFG1
Full Name
Arf-GAP domain and FG repeat-containing protein 1
Weight
58260 MW
Alternative Names
ArfGAP with FG repeats 1; DKFZp686I15205; HIV-1 Rev-binding protein; HRBRev interacting protein; Rab, Rev/Rex activation domain-binding protein; RABMGC116938; RIPMGC116940 AGFG1 HRB, RAB, RIP ArfGAP with FG repeats 1 arf-GAP domain and FG repeat-containing protein 1|HIV-1 Rev-binding protein|Rab, Rev/Rex activation domain-binding protein|arf-GAP domain and FG repeats-containing protein 1|hRIP, Rev interacting protein|nucleoporin-like protein RIP|rev-interacting protein|rev/Rex activation domain-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGFG1, check out the AGFG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGFG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AGFG1 Antibody (RP1071)
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Customer Q&As
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3 Customer Q&As for Anti-AGFG1 Antibody
Question
We are currently using anti-AGFG1 antibody RP1071 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The anti-AGFG1 antibody (RP1071) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-25
Question
Can you help my question with product RP1071, anti-AGFG1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1071 anti-AGFG1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-09
Question
Is a blocking peptide available for product anti-AGFG1 antibody (RP1071)?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
We do provide the blocking peptide for product anti-AGFG1 antibody (RP1071). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-23