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Product Info Summary
| SKU: | PB9365 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband®
View all beta-2 Adrenergic R/ADRB2 Antibodies
SKU/Catalog Number
PB9365
PB0385 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband® catalog # PB9365. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9365)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ADRB2, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9365 is reactive to ADRB2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
46459 MW
Background of beta-2 Adrenergic R/ADRB2
ADRB2, also known as beta-2 adrenergic receptor, is a beta-adrenergic receptor within a cell membrane which reacts with adrenaline (epinephrine) as a hormone or neurotransmitter affecting muscles or organs. It is mapped to 5q32. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca (V)1.2. The genetic variation in the ADRB2 gene may be of major importance for obesity, energy expenditure, and lipolytic ADRB2 function in adipose tissue, at least in women. What’s more, it has been found that activation of ADRB2 receptors can stimulate gamma-secretase activity and beta-amyloid production, and the ADRB2 receptors activator may contribute to beta-amyloid accumulation in AD.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9365 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-ADRB2 Picoband antibody, PB9365, Western blotting
All lanes: AntiADRB2 (PB9365) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
Protein Target Info & Infographic
Gene/Protein Information For ADRB2 (Source: Uniprot.org, NCBI)
Gene Name
ADRB2
Full Name
Beta-2 adrenergic receptor
Weight
46459 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor;ADRB2;ADRB2R, B2AR; ADRB2 ADRB2R, ADRBR, B2AR, BAR, BETA2AR adrenoceptor beta 2 beta-2 adrenergic receptor|adrenergic, beta-2-, receptor, surface|adrenoceptor beta 2 surface|beta-2 adrenoceptor|beta-2 adrenoreceptor|catecholamine receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADRB2, check out the ADRB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband® (PB9365)
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No publications found for PB9365
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Customer Q&As
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15 Customer Q&As for Anti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband®
Question
We are currently using anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-beta 2 Adrenergic Receptor?/ADRB2 antibody (PB9365) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use your anti-beta 2 Adrenergic Receptor?/ADRB2 antibody for WB for human embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
As indicated on the product datasheet, PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-30
Question
I have a question about product PB9365, anti-beta 2 Adrenergic Receptor?/ADRB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-30
Question
My colleagues were satisfied with the WB result of your anti-beta 2 Adrenergic Receptor?/ADRB2 antibody. However we have observed positive staining in fetal brain cell membrane using this antibody. Is that expected? Could you tell me where is ADRB2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
From literature, fetal brain does express ADRB2. Generally ADRB2 expresses in cell membrane. Regarding which tissues have ADRB2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11246467
Brain, Pubmed ID: 3026848, 14702039
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Leukocyte, and Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-20
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
Do you have a BSA free version of anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 available?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-04
Question
Will anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to the expression profile of embryonic kidney, ADRB2 is highly expressed in embryonic kidney. So, it is likely that anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-09-17
Question
We have been able to see staining in rat embryonic kidney. What should we do? Is anti-beta 2 Adrenergic Receptor?/ADRB2 antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Based on literature embryonic kidney does express ADRB2. Based on Uniprot.org, ADRB2 is expressed in squamous epithelium, brain, blood, heart, thyroid, fetal brain, leukocyte prostate, embryonic kidney, among other tissues. Regarding which tissues have ADRB2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11246467
Brain, Pubmed ID: 3026848, 14702039
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Leukocyte, and Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-beta 2 Adrenergic Receptor?/ADRB2 antibody (PB9365)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We do provide the blocking peptide for product anti-beta 2 Adrenergic Receptor?/ADRB2 antibody (PB9365). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-03
Question
you antibody to test anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 on human embryonic kidney for research purposes, then I may be interested in using anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
The products we sell, including anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-19
Question
I see that the anti-beta 2 Adrenergic Receptor?/ADRB2 antibody PB9365 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-04
Question
Would PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
As indicated on the product datasheet, PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-09
Question
My question regards using your anti-beta 2 Adrenergic Receptor?/ADRB2 antibody for positive regulation of transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
C. Miller
Verified customer
Asked: 2014-12-23
Answer
Thank you for your inquiry. This PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody is validated on rat brain tissue, tissue lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-12-23
Question
Is this PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody reactive to the isotypes of ADRB2?
L. Krishna
Verified customer
Asked: 2014-03-26
Answer
The immunogen of PB9365 anti-beta 2 Adrenergic Receptor?/ADRB2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human ADRB2(221-256aa RVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGH), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-03-26
