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Product Info Summary
| SKU: | PB9418 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband®
SKU/Catalog Number
PB9418
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband® catalog # PB9418. Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9418)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ADIPOR1, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9418 is reactive to ADIPOR1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
42616 MW
Background of ADIPOR1
ADIPOR1 is known as Adiponectin receptor protein 1. This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9418 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: Rat Thymus Tissue, Rat Testis Tissue, MCF-7 Whole Cell, A549 Whole Cell
ICC/IF: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Thymus Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: MCF-7 Whole Cell Lysate at 40ug,
Lane 4: A549 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADIPOR1 antigen affinity purified polyclonal antibody (Catalog # PB9418) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADIPOR1 at approximately 43 kDa. The expected band size for ADIPOR1 is at 43 kDa.
Click image to see more details
Figure 2. IF analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418).
ADIPOR1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADIPOR1 Antibody (PB9418) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ADIPOR1 (Source: Uniprot.org, NCBI)
Gene Name
ADIPOR1
Full Name
Adiponectin receptor protein 1
Weight
42616 MW
Superfamily
ADIPOR family
Alternative Names
Adiponectin receptor protein 1;Progestin and adipoQ receptor family member I;ADIPOR1;PAQR1 , TESBP1A;CGI-45; ADIPOR1 ACDCR1, CGI-45, CGI45, PAQR1, TESBP1A adiponectin receptor 1 adiponectin receptor protein 1|progestin and adipoQ receptor family member 1|progestin and adipoQ receptor family member I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADIPOR1, check out the ADIPOR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADIPOR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband® (PB9418)
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Customer Q&As
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5 Customer Q&As for Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband®
Question
Will anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 work for WB with adipose tissue?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
According to the expression profile of adipose tissue, ADIPOR1 is highly expressed in adipose tissue. So, it is likely that anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 will work for WB with adipose tissue.
Boster Scientific Support
Answered: 2020-02-05
Question
I see that the anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The anti-Adiponectin Receptor 1/ADIPOR1 antibody (PB9418) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-01
Question
We want to test anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 on human adipose tissue for research purposes, then I may be interested in using anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
The products we sell, including anti-Adiponectin Receptor 1/ADIPOR1 antibody PB9418, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-19
