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Product Info Summary
| SKU: | A08835-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ADH6 Antibody Picoband®
View all alcohol dehydrogenase 6 Antibodies
SKU/Catalog Number
A08835-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADH6 Antibody Picoband® catalog # A08835-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ADH6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08835-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human ADH6 recombinant protein (Position: E50-K367). Human ADH6 shares 63.3% amino acid (aa) sequence identity with rat ADH6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08835-1 is reactive to ADH6 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39 kDa
Background of alcohol dehydrogenase 6
Alcohol dehydrogenase 6 is an enzyme that in humans is encoded by the ADH6 gene. This gene encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08835-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
FCM: HepG2 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ADH6 using anti-ADH6 antibody (A08835-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH6 antigen affinity purified polyclonal antibody (Catalog # A08835-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH6 at approximately 39 kDa. The expected band size for ADH6 is at 39 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ADH6 antibody (A08835-1).
Overlay histogram showing HepG2 cells stained with A08835-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADH6 Antibody (A08835-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ADH6 (Source: Uniprot.org, NCBI)
Gene Name
ADH6
Full Name
Alcohol dehydrogenase 6
Weight
39 kDa
Superfamily
zinc-containing alcohol dehydrogenase family
Alternative Names
ADH-5; alcohol dehydrogenase 6 (class V); alcohol dehydrogenase 6; aldehyde reductase; EC 1.1.1; EC 1.1.1.1 ADH6 ADH-5 alcohol dehydrogenase 6 (class V) alcohol dehydrogenase 6|aldehyde reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADH6, check out the ADH6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADH6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADH6 Antibody Picoband® (A08835-1)
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