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Product Info Summary
| SKU: | A04090-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-ACF1/BAZ1A Antibody Picoband®
SKU/Catalog Number
A04090-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACF1/BAZ1A Antibody Picoband® catalog # A04090-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ACF1/BAZ1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04090-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ACF1/BAZ1A recombinant protein (Position: E1033-H1536).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04090-1 is reactive to BAZ1A in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
203 kDa
Calculated molecular weight
211344 MW
Background of ACF1
Bromodomain adjacent to zinc finger domain protein 1A is a protein that in humans is encoded by the BAZ1A gene. The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04090-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: monkey COS-7 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse testis tissue
ICC/IF: MCF-7 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ACF1/BAZ1A using anti-ACF1/BAZ1A antibody (A04090-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACF1/BAZ1A antigen affinity purified polyclonal antibody (Catalog # A04090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACF1/BAZ1A at approximately 203 kDa. The expected band size for ACF1/BAZ1A is at 179 kDa.
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Figure 2. IF analysis of ACF1/BAZ1A using anti-ACF1/BAZ1A antibody (A04090-1).
ACF1/BAZ1A was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ACF1/BAZ1A Antibody (A04090-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-ACF1/BAZ1A antibody (A04090-1).
Overlay histogram showing HepG2 cells stained with A04090-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACF1/BAZ1A Antibody (A04090-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For BAZ1A (Source: Uniprot.org, NCBI)
Gene Name
BAZ1A
Full Name
Bromodomain adjacent to zinc finger domain protein 1A
Weight
211344 MW
Superfamily
WAL family
Alternative Names
Muscarinic acetylcholine receptor M1; CHRM1 BAZ1A ACF1, WALp1, WCRF180, hACF1 bromodomain adjacent to zinc finger domain 1A bromodomain adjacent to zinc finger domain protein 1A|ATP-dependent chromatin remodeling protein|ATP-utilizing chromatin assembly and remodeling factor 1|CHRAC subunit ACF1|hWALp1|williams syndrome transcription factor-related chromatin-remodeling factor 180
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAZ1A, check out the BAZ1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAZ1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACF1/BAZ1A Antibody Picoband® (A04090-1)
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