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Product Info Summary
| SKU: | P12111 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Acetylated Histone H1 (Lys25) H1FOO Antibody
SKU/Catalog Number
P12111
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Acetylated Histone H1 (Lys25) H1FOO Antibody (Catalog# P12111). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetylated Histone H1 (Lys25) H1FOO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P12111)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Histone H1 around the acetylated site of Lys25. Immunogen sequence location: 131 - 180
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P12111 is reactive to H1FOO in Human, Monkey
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
35813 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P12111 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H1 (Acetyl-Lys25) Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H1 (Acetyl-Lys25) Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For H1FOO (Source: Uniprot.org, NCBI)
Gene Name
H1FOO
Full Name
Histone H1oo
Weight
35813 MW
Superfamily
histone H1/H5 family
Alternative Names
H1FOO;H1OO;OSH1;Histone H1oo;Oocyte-specific histone H1;Oocyte-specific linker histone H1;osH1;H1K25AC
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H1FOO, check out the H1FOO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H1FOO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acetylated Histone H1 (Lys25) H1FOO Antibody (P12111)
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No publications found for P12111
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Customer Q&As
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1 Customer Q&As for Anti-Acetylated Histone H1 (Lys25) H1FOO Antibody
Question
We are currently using anti-Acetylated Histone H1 (Lys25) antibody P12111 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The anti-Acetylated Histone H1 (Lys25) antibody (P12111) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
