Product Info Summary
SKU: | A10397 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-17 beta-HSD11 HSD17B11 Antibody
View all hydroxysteroid (17-beta) dehydrogenase 11 Antibodies
SKU/Catalog Number
A10397
Size
100μl
Form
Liquid
Description
Boster Bio Anti-17 beta-HSD11 HSD17B11 Antibody catalog # A10397. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-17 beta-HSD11 HSD17B11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10397)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DHRS8. AA range:71-120
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10397 is reactive to HSD17B11 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
32936 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10397 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using 17beta-HSD11 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B11 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B11
Full Name
Estradiol 17-beta-dehydrogenase 11
Weight
32936 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
Estradiol 17-beta-dehydrogenase 11;1.1.1.62;17-beta-hydroxysteroid dehydrogenase 11;17-beta-HSD 11;17bHSD11;17betaHSD11;17-beta-hydroxysteroid dehydrogenase XI;17-beta-HSD XI;17betaHSDXI;Cutaneous T-cell lymphoma-associated antigen HD-CL-03;CTCL-associated antigen HD-CL-03;Dehydrogenase/reductase SDR family member 8;Retinal short-chain dehydrogenase/reductase 2;retSDR2;Short chain dehydrogenase/reductase family 16C member 2;HSD17B11;DHRS8, PAN1B, SDR16C2;PSEC0029, UNQ207/PRO233; HSD17B11 17-BETA-HSD11, 17-BETA-HSDXI, 17BHSD11, DHRS8, PAN1B, RETSDR2, SDR16C2 hydroxysteroid 17-beta dehydrogenase 11 estradiol 17-beta-dehydrogenase 11|17-beta-hydroxysteroid dehydrogenase type XI|CTCL tumor HD-CL-03|CTCL-associated HD-CL-03|cutaneous T-cell lymphoma-associated HD-CL-03|dehydrogenase/reductase SDR family member 8|retinal short-chain dehydrogenase/reductase 2|short chain dehydrogenase/reductase family 16C member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B11, check out the HSD17B11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-17 beta-HSD11 HSD17B11 Antibody (A10397)
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No publications found for A10397
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Customer Q&As
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5 Customer Q&As for Anti-17 beta-HSD11 HSD17B11 Antibody
Question
I see that the anti-17 beta-HSD11 antibody A10397 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-17 beta-HSD11 antibody A10397. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
Does A10397 anti-17 beta-HSD11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can see on the product datasheet, A10397 anti-17 beta-HSD11 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-17 beta-HSD11 antibody for WB for human teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human teratocarcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
You can see on the product datasheet, A10397 anti-17 beta-HSD11 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-17 beta-HSD11 antibody A10397 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The anti-17 beta-HSD11 antibody (A10397) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-12