Alkaline Phosphatase Conjugated Streptavidin

Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity. Cited in 7 publication(s).

Product Info Summary

SKU: BA1027
Size: 0.1ml
Application: ELISA, IHC, WB

Product NameAlkaline Phosphatase Conjugated Streptavidin
SKU/Catalog NumberBA1027
Pack Size0.1 ml
FormConcentrated, Liquid
Concentration1 mg/ml
Product DescriptionStreptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-peroxidase can form a complex which composed by about one hundred peroxidase and fifty streptavidin. And lots of enzymes can ensure the streptavidin-peroxidase with high sensitivity.
HostEgg white avidin
Contents0.1 mg of Alkaline Phosphatase conjugated specific antibody; 0.05 M Tris buffer, pH 8.0, containing 1% BSA, 1 mM MgCl2, 50% glycerol, with 15 mM sodium azide as a preservative.
SpecificityThis Alkaline Phosphatase conjugated antibody is specific for Strept Avidin
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsDirect ELISA, IHC, WB
Cite This ProductAlkaline Phosphatase Conjugated Streptavidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1027)

Validation Images & Assay Conditions

Hello CJ!

BA1027 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat

Colorimetric sensing of alkaline phosphatase and α-fetoprotein based on the photoinduced oxidase activity of fluorescein

A signal amplification strategy for prostate specific antigen detection via releasing oxidase-mimics from coordination nanoparticles by alkaline phosphatase

Split-type electrochemiluminescent gene assay platform based on gold nanocluster probe for human papillomavirus diagnosis

Reducing background absorbance via a double-lock strategy for detection of alkaline phosphatase and α-fetoprotein

Sun C,Shi Y,Tang M,Hu X,Long Y,Zheng H.A signal amplification strategy for prostate specific antigen detection via releasing oxidase-mimics from coordination nanoparticles by alkaline phosphatase.Talanta.2020 Jun 1;213:120827.doi:10.1016/j.talanta.2020.12
Species: -
BA1027 usage in article: APP:ALP LINKED IMMUNOASSAY, SAMPLE:NANOPARTICLE, DILUTION:NA

Wang JK, Zhao BS, Wang M, Liu CY, Li YQ, Ma QT, Li PF, Wang TS, Wang CG, Zhou YM. Anti-tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway. Chin J Integr Med. 2020 Nov 10. doi: 10.1007/s11655-020-3433-8. Ep
Species: Mouse
BA1027 usage in article: APP:NOT MENTIONED, SAMPLE:DCS, DILUTION:NA

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