Activin-B Human Recombinant Protein, Active

Product Name

Activin-B Human Recombinant Protein, Active

View all Activin B/Inhibin beta B recombinant proteins

SKU/Catalog Number

PROTP09529

Size

2ug, 10ug, 1mg

Description

Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C₆₁₅H₉₁₀N₁₇₈O₁₇₇S₁₂). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Storage & Handling

Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

Activin-B Human Recombinant Protein, Active (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09529)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 1mg/ml solution in 0.05M Tris-HCl buffer pH 7.4.

Purity

Greater than 97.0% as determined by Analysis by SDS-PAGE.

Predicted MW

45.122kDa

Reconstitution

It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 less than 5ng/ml is required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Reconstitution

It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For INHBB (Source: Uniprot.org, NCBI)

Alternative Names

Inhibin beta B (activin AB beta polypeptide); Inhibin; beta-2; Activin beta-B chain; MGC157939 INHBB inhibin subunit beta B inhibin beta B chain|Inhibin, beta-2|activin AB beta polypeptide|activin beta-B chain|inhibin beta B subunit

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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