SLC34A1 (NM_003052) Human Over-expression Lysate

Product Name

SLC34A1 (NM_003052) Human Over-expression Lysate

See all SLC34A1 products

SKU/Catalog Number

LS006569

Size

100 µg

Description

Transient overexpression lysate of solute carrier family 34 (sodium phosphate), member 1 (SLC34A1), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

SLC34A1 (NM_003052) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006569)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SLC34A1 (Source: Uniprot.org, NCBI)

Alternative Names

FRTS2; Na(+)/Pi cotransporter 2A; Na(+)-dependent phosphate cotransporter 2A; naPi-2a; NAPI-3; NPT2NPHLOP1; NPTIIa; renal sodium-dependent phosphate transporter; SLC11; SLC17A2Na+-phosphate cotransporter type II; Sodium/phosphate cotransporter 2A; sodium/phosphate co-transporter; sodium-dependent phosphate transport protein 2A; Sodium-phosphate transport protein 2A; solute carrier family 17 (sodium phosphate), member 2; solute carrier family 34 (sodium phosphate), member 1; Solute carrier family 34 member 1 SLC34A1 FRTS2, HCINF2, NAPI-3, NPHLOP1, NPT2, NPTIIa, SLC11, SLC17A2 solute carrier family 34 member 1 sodium-dependent phosphate transport protein 2A|Na(+)-dependent phosphate cotransporter 2A|Na(+)/Pi cotransporter 2A|Na+-phosphate cotransporter type II|naPi-2a|renal sodium-dependent phosphate transporter|sodium-phosphate transport protein 2A|sodium/phosphate co-transporter|sodium/phosphate cotransporter 2A|solute carrier family 17 (sodium phosphate), member 2|solute carrier family 34 (sodium phosphate), member 1|solute carrier family 34 (type II sodium/phosphate cotransporter), member 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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