Fully customizable Gene Expression Analysis

Gene Expression Analysis Services

From Nucleic Acid purification to a comprehensive bioinformatic analysis report, Boster’s Gene Expression Analysis services can cover all of your NGS requirements. Explore differentially expressed genes, functions or pathways in your samples under any specific developmental stages or conditions.

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Gene Expression Analysis Service Overview

Boster Bio offers a complete Gene Expression Analysis Service to turn your samples into insight.

We support your entire project, or any specific part, by covering every aspect of the gene expression analysis workflow, from nucleic acid extraction to bioinformatics analysis. Our expert scientists utilize the latest technologies in conjunction with decades of experience to deliver high-quality gene expression profiling solutions.

Our Services include:

  • Nucleic Acid extraction and purification
  • MicroRNA capture and isolation
  • cDNA synthesis and library generation
  • Target enrichment or depletion
  • Sequencing by Synthesis
  • Bioinformatic analysis and reporting
dna-transcription-translation

What is Gene Expression and why is it important?

Gene expression is the process by which information encoded in a gene is transcribed into a functional gene product such as proteins or functional RNA’s, causing a phenotypic effect or expression within the system. Gene expression can be regulated at various levels of transcription, mRNA development and translation forming the basis of cell development and differentiation.

Gene expression analysis can be used to distinguish between different disease states, discover the genes or pathways involved in disease development, or to investigate the functional capabilities of a new therapeutic drug. It can uncover new molecular reporters and novel drug targets, predict a patient’s disease classification, and help optimize treatments.

How to Order Boster’s Gene Expression Analysis Service

As easy as 1, 2, 3.

1. Free Project Consultation with our Expert Scientists


2. Send Samples



3. Receive a Comprehensive Analysis Report


Gene Expression Analysis Services Overview

Nucleic Acid Extraction

Extract genomic and/or plasmid DNA from your sample without worry. Our cutting-edge technologies are used to purify your sample from contaminants and reduce the probability of errors.

  • High throughput sample processing for biopharma research
  • High yield, high purity DNA and RNA isolation
  • Safe, stable sample collection for pathogen inactivation
  • Mixed population or single species from any sample type
  • Extraction methodology tailored to each sample type

Deliverables

  • Purified Nucleic Acid (RNA or DNA)
  • Plate Layout (if nucleic acid is returned in a 96-well plate
  • QC Purity Report

Total DNA or RNA Sequencing Service

Boster Bio’s Gene Expression service provides an end-to-end solution for all your DNA or RNA sequencing requirements. From nucleic acid purification, library preparation, sequencing and analysis we have your sequencing needs covered.

Boster’s Probe-Free technology uses the input RNA as a template to drive the depletion of the reverse transcribed cDNA from the highly abundant sequences. This allows the depletion to be probe-free and universally compatible with RNA from any organism.

  • Compatible with all samples including low input and FFPE samples
  • Workflow compatible with any organism type
  • Unbiased gene expression data with probe-free technology

Deliverables:

  • Total RNA-Seq: mRNAs and long-noncoding RNAs will be sequenced, ribosomal RNAs will be removed during library preparation.
  • 3’mRNA-Seq: only mRNAs will be sequenced; library preparation protocol involves poly(A) enrichment
  • Comprehensive Bioinformatics gene expression report

miRNA Sequencing

microRNA (miRNA) are small non-coding RNAs that are very short in length (17-25nt) which are highly conserved and expressed in a tissue specific manner. miRNA’s are important in transcriptional and post-transcriptional gene relation and have been found to be instrumental in some specific cancer progression and hereditary diseases.

Boster Bio offer an efficient microRNA capture and detection solution generating more reads.

  • Compatible with cfRNA from plasma and serum samples
  • Isolation followed by cfRNA-seq library prep
  • microRNA expression data analysis report

We promise You a concierge sourcing experience

Scholars serving scholars

It is our project concierges' mission to make your project experience as smooth and memorable as possible. They are subject matter experts who are easily accessible around the clock, always happy to help you solve problems, make recommendations and sort through options.

Book a meeting with your project concierge

Gene Expression Analysis for any sample and species

FFPE

Enzymatic Reactions

Samples in Trizol®

Cells

Tissues

Biological Fluids

Insect Vectors

Environmental

Infectious Materials

Advantages of using Boster’s Gene Expression Analysis Service

End-to-end
and custom service

Optimised sample
processing workflows

Cutting-edge
bioinformatics

Competitive
pricing

Project concierge
to help at every step of the way

Fast sample
turnaround

Sample Report

FAQs

Q1. What kind of samples can be used with this service?

Boster Bio’s Gene Expression service can be used for any sample and organism type. For more information on the types of samples and species see this link

Q2. How do we prepare our samples for NGS sequencing?

Our Project Concierge will work with you to determine what is required for your NGS service. Samples can be prepared and sent following our Sample Preparation Guide.

Q3. What is Transcriptome analysis?

Transcriptome analysis is the method of analysing RNA via RNA-seq to enable researchers to characterize transcriptional activity. Whole transcriptome analysis detects both coding and non-coding RNA and allows the researcher to focus on a subset of relevant target genes and transcripts, or profile thousands of genes at once to create a global picture of cell function.

Q4. What is the minimum amount of RNA/DNA that you can process?

We ask for 500—1000 ng of total RNA that has been treated with DNase I. If you are not able to provide this much, the absolute minimum is 150 ng at the concentration of 12.5 ng/ul. We do have protocols that allow us to work with less than 100 ng RNA; however we will have to address such situations on a case by case basis. For more information on Sample requirements please see our sample guidelines page.

Q5. How does the Probe-Free technology work?

Boster Bio’s Probe-Free technology uses the input RNA as a template to drive the depletion of the reverse transcribed cDNA from the highly abundant sequences. This allows the depletion to be probe-free and universally compatible with RNA from any organism. Learn more about the probe-free depletion from this feature in Nature

Q6. Why is Boster so much more efficient at capturing miRNA than other suppliers?

We have developed and optimized extraction kits to make sure that miRNA is captured efficiently, such as for example Quick-cfRNA serum and plasma kit. For more information on how Boster can efficiently capture your miRNA, please contact our Project Concierge

Q7. What is the usual turnaround time for Total DNA or RNA-seq services?

Turnaround time is 8–12 weeks for 50 samples or less. If it is more than 50 samples, turnaround time might be longer.