Product Info Summary
SKU: | RCOV13 |
---|---|
Size: | 5ug, 10ug |
Origin Species: | Mouse |
Source: | Sf9 insect cells using baculovirus |
Product info
Product Name
Recombinant Human METTL3/METTL14 Complex
SKU/Catalog Number
RCOV13
Size
5ug, 10ug
Description
Full-length recombinant human METTL3/METTL14 complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The METTL3 and METTL14 gene accession numbers are NM_019852 and NM_020961.
Storage & Handling
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. (Ship on dry ice.)
Cite This Product
Recombinant Human METTL3/METTL14 Complex (Boster Biological Technology, Pleasanton CA, USA, Catalog # RCOV13)
Form
Liquid
Formulation
Recombinant proteins stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Purity
The purity of METTL3/METTL14 complex was determined to be >90% by densitometry, approx. MW 100 kDa (METTL3) and 84 kDa (METTL14).
Assay dilution & Images
Validation Images & Assay Conditions
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The specific activity of METTL3/METTL14 was determined to be 750 pmol /min/mg as per activity assay protocol.
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The purity of METTL3/METTL14 complex was determined to be >90% by densitometry, approx. MW 100 kDa (METTL3) and 84 kDa (METTL14).
Specific Publications For Recombinant Human METTL3/METTL14 Complex (RCOV13)
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