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Product Info Summary
| SKU: | DZ33921-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
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Product info
Product Name
Anti-Fruit fly CG6353 Antibody
SKU/Catalog Number
DZ33921-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-CG6353 Antibody catalog # DZ33921-1. This antibody reacts with Fruit fly.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fruit fly CG6353 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33921-1)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
DZ33921-1 is reactive to Q9VD92 in Fruit fly
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Validation Images & Assay Conditions
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Figure 1. IF analysis of CG6353 using anti-CG6353 antibody (DZ33921-1).
CG6353 was detected in a paraffin-embedded section of drosophila larvae tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 4% horse serum. The tissue section was then incubated with rabbit anti-CG6353 Antibody (DZ33921-1) at 1:100 dilution overnight at 4°C. Anti-Rb-A488 was used as secondary antibody at 1:1000 dilution. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Q9VD92 (Source: Uniprot.org, NCBI)
Gene Name
Q9VD92
Full Name
Weight
For more info on Q9VD92, check out the Q9VD92 Infographic
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In this infographic, you will see the following information for Q9VD92: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fruit fly CG6353 Antibody (DZ33921-1)
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Customer Reviews
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1 Reviews For Anti-Fruit fly CG6353 Antibody
0
Immunofluorescence for Anti-CG6353 Antibody--Amin Ghabrial Pathology and Cell Biology Columbia University Principal Investigator
Excellent
Source: Biocompare.com
"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."
| Application | Immunofluorescence |
|---|---|
| Sample | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Drosophila larvae |
| Primary Incubation | 1:100 at 4 degrees overnight |
| Blocking Agent | 4% horse serum |
| Secondary Incubation | 1:1000 anti-Rb-A488 |
| Tertiary Incubation | N/A |
| Detection | IF |
| Results Summary | Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear. |
Amin Ghabrial
Verified customer
Submitted 2021-11-29
