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Product Info Summary
| SKU: | LS080119 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
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Product info
Product Name
PIF1 (NM_025049) Human Over-expression Lysate
SKU/Catalog Number
LS080119
Size
100 µg
Description
Transient overexpression lysate of PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
PIF1 (NM_025049) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080119)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PIF1 Human Over-expression Lysates NM_025049.
Western blot validation of overexpression lysate (LS080119) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PIF1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PIF1 (Source: Uniprot.org, NCBI)
Gene Name
PIF1
Full Name
ATP-dependent DNA helicase PIF1
Weight
69.799kDa
Superfamily
helicase family
Alternative Names
C15orf20; PIF; PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae); PIF1 5-to-3 Prime DNA helicase homolog; PIF1 DNA helicase; PIF1/RRM3 DNA helicase-like protein; RRM3 DNA helicase-like protein PIF1 C15orf20, PIF PIF1 5-to-3 DNA helicase ATP-dependent DNA helicase PIF1|DNA repair and recombination helicase PIF1|PIF1 5-to-3 DNA helicase homolog|PIF1/RRM3 DNA helicase-like protein|petite integration frequency 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PIF1, check out the PIF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PIF1 (NM_025049) Human Over-expression Lysate (LS080119)
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