Product Info Summary
SKU: | LS057532 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of nuclear fragile X mental retardation protein interacting protein 2 (NUFIP2) |
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Product info
Product Name
NUFIP2 (NM_020772) Human Over-expression Lysate
SKU/Catalog Number
LS057532
Size
100 µg
Description
Transient overexpression lysate of nuclear fragile X mental retardation protein interacting protein 2 (NUFIP2)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
NUFIP2 (NM_020772) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS057532)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls057532 nufip2 human over expression lysates nm 020772 wb testing 1 ls057532 nufip2 human over expression lysates nm 020772 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls057532-nufip2-human-over-expression-lysates-nm-020772-wb-testing-1.jpg)
Click image to see more details
NUFIP2 Human Over-expression Lysates NM_020772.
Western blot validation of overexpression lysate (LS057532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NUFIP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for NUFIP2 (Source: Uniprot.org, NCBI)
Gene Name
NUFIP2
Full Name
Nuclear fragile X mental retardation-interacting protein 2
Weight
76.121kDa
Alternative Names
182-FIP; Cell proliferation-inducing gene 1 protein; FIP-82; FLJ10976; FMRP-interacting protein 2; Fragile X Mental Retardation Protein; KIAA1321PIG1,82-FIP82-kD FMRP Interacting Protein; MGC117262; nuclear fragile X mental retardation protein interacting protein 2; nuclear fragile X mental retardation-interacting protein 2; proliferation-inducing gene 1,82 kDa FMRP-interacting protein NUFIP2 182-FIP, 82-FIP, FIP-82, PIG1 nuclear FMR1 interacting protein 2 nuclear fragile X mental retardation-interacting protein 2|82 kDa FMRP-interacting protein|82-kD FMRP Interacting Protein|FMRP-interacting protein 2|Fragile X Mental Retardation Protein|NUFIP2, FMR1 interacting protein 2|cell proliferation-inducing gene 1 protein|nuclear fragile X mental retardation protein interacting protein 2|proliferation-inducing gene 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NUFIP2, check out the NUFIP2 Infographic
![NUFIP2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUFIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NUFIP2 (NM_020772) Human Over-expression Lysate (LS057532)
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