N acetyl transferase 5 (NAA20) (NM_016100) Human Over-expression Lysate

Product Name

N acetyl transferase 5 (NAA20) (NM_016100) Human Over-expression Lysate

See all N acetyl transferase 5 products

SKU/Catalog Number

LS051126

Size

100 µg

Description

Transient overexpression lysate of N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

N acetyl transferase 5 (NAA20) (NM_016100) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051126)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NAA20 (Source: Uniprot.org, NCBI)

Alternative Names

dJ1002M8.1; dJ1175I6.2 (N-terminal acetyltransferase complex ard1 subunit); EC 2.3.1.88; homolog); N(alpha)-acetyltransferase 20, NatB catalytic subunit; N-acetyltransferase 3 homolog; N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae); N-acetyltransferase 5 (GCN5-related, putative); N-acetyltransferase 5; N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae; N-alpha-acetyltransferase 20, NatB catalytic subunit; NAT3N-terminal acetyltransferase B complex catalytic subunit NAT5; NAT5dJ1002M8.1 (N-terminal acetyltransferase complex ard1 subunit); NatB complex subunit NAT5; N-t NAA20 NAT3, NAT3P, NAT5, NAT5P, dJ1002M8.1 N-alpha-acetyltransferase 20, NatB catalytic subunit N-alpha-acetyltransferase 20|N-acetyltransferase 3 homolog|N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae)|N-acetyltransferase 5 (GCN5-related, putative)|N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae, homolog)|N-terminal acetyltransferase B complex catalytic subunit NAA20|N-terminal acetyltransferase B complex catalytic subunit NAT5|N-terminal acetyltransferase complex ARD1 subunit|methionine N-acetyltransferase|natB catalytic subunit|natB complex subunit NAT5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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