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Product Info Summary
| SKU: | LS004642 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of myosin ID (MYO1D) |
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Product info
Product Name
MYO1D (NM_015194) Human Over-expression Lysate
SKU/Catalog Number
LS004642
Size
100 µg
Description
Transient overexpression lysate of myosin ID (MYO1D)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
MYO1D (NM_015194) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004642)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MYO1D Human Over-expression Lysates NM_015194.
Western blot validation of overexpression lysate (LS004642) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MYO1D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MYO1D (Source: Uniprot.org, NCBI)
Gene Name
MYO1D
Full Name
Unconventional myosin-Id
Weight
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
KIAA0727myr4; myosin ID; myosin-I gamma; myosin-Id MYO1D PPP1R108, myr4 myosin ID unconventional myosin-Id|myosin-I gamma|protein phosphatase 1, regulatory subunit 108
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MYO1D, check out the MYO1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYO1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MYO1D (NM_015194) Human Over-expression Lysate (LS004642)
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