Mouse recombinant IL-17F (Interleukin-17F) protein, AF

IL-17F protein, Mouse

Interleukin 17F (IL-17F) predicts a molecular mass of 30.1 kDa, is a cytokine of innate and adaptive immune system involved in maintenance of tissue integrity and antimicrobial host defense against infection by inducing the expression of genes that encode other proinflammatory cytokines, such as tumor necrosis factor, interleukin 1, interleukin 6 and some members of the colony-stimulating factor family.

Product Info Summary

SKU: PROTQ7TNI7-2
Size: 5ug,20ug,100ug
Origin Species: Mouse
Source: Escherichia coli
Application: Cell Culture

Product Name

Mouse recombinant IL-17F (Interleukin-17F) protein, AF

View all IL-17F recombinant proteins

SKU/Catalog Number

PROTQ7TNI7-2

Size

5ug,20ug,100ug

Tag

His Tag (C-term)

Description

Interleukin 17F (IL-17F) predicts a molecular mass of 30.1 kDa, is a cytokine of innate and adaptive immune system involved in maintenance of tissue integrity and antimicrobial host defense against infection by inducing the expression of genes that encode other proinflammatory cytokines, such as tumor necrosis factor, interleukin 1, interleukin 6 and some members of the colony-stimulating factor family.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Mouse recombinant IL-17F (Interleukin-17F) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ7TNI7-2)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, pH 4.5. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

18.045kDa

Molecular weight

The protein has a calculated MW of 15.82 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <100 ng/mL.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

MRKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPREFQNRSSSPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILVLRREPQGCSNSFRLEKMLLKVGCTCVKPIVHQAA with polyhistidine tag at the C-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For IL17F (Source: Uniprot.org, NCBI)

Gene Name

IL17F

Full Name

Interleukin-17F

Weight

18.045kDa

Superfamily

IL-17 family

Alternative Names

C87042 IL17F CANDF6, IL-17F, ML-1, ML1 interleukin 17F interleukin-17F|cytokine ML-1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IL17F, check out the IL17F Infographic

IL17F infographic

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In this infographic, you will see the following information for IL17F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTQ7TNI7-2

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