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Product Info Summary
| SKU: | FEK0394 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit
See all IL-1 beta/IL-1F2 products
SKU/Catalog Number
FEK0394
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Mouse IL1B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0394)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V118-S269
Sensitivity
<1 pg/ml
Assay Range
12.5 pg/ml - 800 pg/ml
Standard Dilution Instructions
See datasheet of FEK0394 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0394 is reactive to IL1B in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0394 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 beta/IL-1F2
Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0394-CAP | Anti-Mouse IL-1 beta/IL-1F2 Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0394-ST | Mouse IL-1 beta/IL-1F2 Standard | 2 | 10 ng/tube |
| FEK0394-H | HRP Linked Anti-Mouse IL1B Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 12.5 | 25 | 50 | 100 | 200 | 400 | 800 |
| O.D. | 0.030 | 0.065 | 0.123 | 0.212 | 0.336 | 0.609 | 1.031 | 1.898 |
Data Example Images
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Human IL-1 beta PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 211 | 483 | 36 | 217 | 481 |
| Standard deviation | 1.74 | 10.33 | 34.29 | 2.19 | 14.75 | 35.59 |
| CV (%) | 5.3% | 4.9% | 7.1% | 6.1% | 6.8% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 31 | 32 | 32 | 1.11 | 3.4% |
| Sample 2 | 211 | 206 | 203 | 208 | 207 | 2.91 | 1.4% |
| Sample 3 | 483 | 452 | 433 | 430 | 449 | 21.1 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30.748kDa
Superfamily
IL-1 family
Alternative Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (FEK0394)
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