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Polymerase Chain Reaction (PCR) Technical Resources
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Download PCR eBookTechnical Resources Overview
PCR Principle
Molecular biology has long been the basis for the understanding of each individual step in the biology central dogma: DNA replication, DNA transcription into RNA, and RNA translation into proteins. Manipulation and investigation of genetic material is done through various techniques including traditional molecular cloning (library construction), Polymerase Chain Reaction (PCR), and gel electrophoresis. These molecular biology techniques have various broad and useful applications in our scientific community, so understanding the fundamental principles will be useful in your journey.
Click Here for MorePCR Sample Preparation
Every experiment begins with sample preparation. We have included the standard operating procedures for the basic techniques of molecular biology. If you need instructions for DNA and RNA extraction and purification or for molecular cloning (DNA library preparation), you have come to the right place. Use our step-by-step guides as reference, and be sure to check the product datasheets for specific details.
Click Here for MorePCR Protocols
Your experiment requires a solid protocol. We have provided guidelines for designing primers and performing polymerase chain reaction. If you're looking for protocols of PCR, reverse transcription, and qRT-PCR, we've posted step-by-step guides for your reference. Be sure to check the product datasheets for specific details. Following an optimized protocol will lead to better and more reproducible data results.
Click Here for MorePCR Troubleshooting Tips
An unsuccessful experiment can be disheartening, but we have provided here a simplified table for solving your troubles. If you encounter problems such as low yields or contamination when extracting DNA and RNA, we have some common solutions to help. If you have no idea why your PCR experiment failed, then check our troubleshooting table for advice on how to fix your specific problem.
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ELISA Technical Resource Center
Western blot, IHC, and ELISA all require different ways of preparing a sample, and nearly every sample type requires specialized treatment in order to produce high-quality, consistent results. Check out the ELISA sample preparation guides to learn ho...
See MorePCR Protocols
Learn stepwise PCR protocols from reagent preparation to data analysis. Check out their differences to learn how they compare and their advantages and disadvantage.
See MoreFlow Cytometry Technical Resource Center
Click Here for More Flow Cytometry Sample Preparation Every flow cytometry (or FACS) experiment begins with sample preparation. Click Here for More Flow Cytometry Technical Blogs Here is our ever growing archive of technical blog articles related to...
See MorePCR and Molecular Biology Fundamental Principles
The DNA strand used to produce the complementary strand is referred to as the template strand, which is used to synthesize complementary strands of each parental strand. These molecules are responsible for giving information to cells of each organism...
See MorePCR Sample Preparation
PCR sample preparation is very crucial for reliable results. It includes accurate extraction and purification of DNA or RNA to the library preparation and screening.
See MoreWestern Blotting Technical Resource Center
Check out our Western blotting sample preparation resources to learn how to prepare your sample type for Western blotting. Click Here for More Western Blotting Sample Preparation Every immunoassay protocol begins with sample preparation. Click Here f...
See MorePCR Troubleshooting Tips
We hope that you will find the information beneficial to you and useful as a reference guide in troubleshooting any problems you may encounter. If you ever need more assistance with your experiments, please contact the Boster Support Team by email at...
See MoreImmunohistochemistry (IHC) Resource Center
The aim of these resources is to provide a comprehensive overview of the immunohistochemistry (IHC) resources available for use in research. We have selected a number of key IHC protocols, troubleshootin,g, and optimization tips that are widely used by researchers, as well as some useful tools and techniques that can be applied to specific types of tissue or cells.
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