Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate

Product Name

Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate

See all Ku70/XRCC6 products

SKU/Catalog Number

LS002547

Size

100 µg

Description

Transient overexpression lysate of X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002547)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for XRCC6 (Source: Uniprot.org, NCBI)

Alternative Names

5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; D22S731; EC 3.6.4.-; EC 4.2.99.-; G22P1; G22P1Ku70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; ML8; ML8,70 kDa subunit; thyroid-lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; TLAAD22S671; X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6; X-ray repair cross-compleme XRCC6 CTC75, CTCBF, G22P1, KU70, ML8, TLAA X-ray repair cross complementing 6 X-ray repair cross-complementing protein 6|5-dRP lyase Ku70|5-deoxyribose-5-phosphate lyase Ku70|70 kDa subunit of Ku |ATP-dependent DNA helicase 2 subunit 1|ATP-dependent DNA helicase II, 70 kDa subunit|CTC box binding factor 75 kDa subunit|DNA repair protein XRCC6|Ku auto p70 subunit|Ku auto, 70kDa|X-ray repair complementing defective repair in Chinese hamster cells 6|lupus Ku auto protein p70|thyroid auto 70kD (Ku )|thyroid auto 70kDa (Ku )|thyroid-lupus auto p70

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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