Product Info Summary
SKU: | PROTQ63264 |
---|---|
Size: | 2ug, 10ug, 1mg |
Origin Species: | Rat |
Source: | Escherichia coli |
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Product info
Product Name
IL-1-beta Interleukin-1 beta Rat Recombinant Protein
View all IL-1 beta/IL-1F2 recombinant proteins
SKU/Catalog Number
PROTQ63264
Size
2ug, 10ug, 1mg
Description
Interleukin-1b Rat Recombinant produced in E. coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17.3 kDa. The IL-1b is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
IL-1-beta Interleukin-1 beta Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ63264)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from 0.2um filtered concentrated solution in PBS, pH 7.4.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Predicted MW
30.748kDa
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin 1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
MVPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSF VQGETSNDKIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKK KMEKRFVFNKIEVKTKVEFESAQFPNWYISTSQAEHRPVFLGNSNGRD IVDFTMEPVSS
Biological Activity
The ED50 was found to be less than 0.1ng/ml, determined by the dose dependent proliferation of mouse D10S cells, corresponding to a specific activity of 10,000,000 units/mg.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin 1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
Click image to see more details
Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30.748kDa
Superfamily
IL-1 family
Alternative Names
Catabolin; Lymphocyte-activating factor (LAF); Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Mononuclear Cell Factor (MCF); IL1F2; IL-1 beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
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In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IL-1-beta Interleukin-1 beta Rat Recombinant Protein (PROTQ63264)
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