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Product Info Summary
| SKU: | EK2025 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human SMPD1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2025
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human SMPD1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SMPD1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human SMPD1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2025)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: L47-C631
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2025 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2025 is reactive to SMPD1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK2025 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SMPD1
Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2025-CAP | Anti-Human SMPD1 Pre-coated 96-well strip microplate | 1 |
| EK2025-ST | Human SMPD1 Standard | 2 vials, 10 ng/tube |
| EK2025-DA | Human SMPD1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.032 | 0.074 | 0.140 | 0.230 | 0.369 | 0.636 | 1.058 | 1.702 |
Data Example Images
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Human SMPD1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SMPD1, Dilution ratio of 1:4, concentration in serum and plasma is around 6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SMPD1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 330 | 1190 | 4173 | 351 | 1215 | 4524 |
| Standard deviation | 14.19 | 61.88 | 191.96 | 21.41 | 71.69 | 334.78 |
| CV (%) | 4.3% | 5.2% | 4.6% | 6.1% | 5.9% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SMPD1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 330 | 332 | 308 | 305 | 319 | 15.3 | 4.8% |
| Sample 2 | 1190 | 1185 | 1201 | 1218 | 1199 | 43.15 | 0.9% |
| Sample 3 | 4173 | 4545 | 4622 | 4796 | 4534 | 231.23 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For SMPD1 (Source: Uniprot.Org, NCBI)
Gene Name
SMPD1
Full Name
Sphingomyelin phosphodiesterase
Weight
Superfamily
acid sphingomyelinase family
Alternative Names
Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal SMPD1 ASM, ASMASE, NPD sphingomyelin phosphodiesterase 1 sphingomyelin phosphodiesterase|Niemann-Pick type A/B|acid sphingomyelinase|sphingomyelin phosphodiesterase 1, acid lysosomal
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SMPD1, check out the SMPD1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SMPD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human SMPD1 ELISA Kit PicoKine® (EK2025)
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Customer Q&As
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7 Customer Q&As for Human SMPD1 ELISA Kit PicoKine®
Question
Q: is it okay to use EDTA plasma as samples in Human SMPD1 Picokine® ELISA Kit (Catalog # EK2025)?
Z. Allen
Verified customer
Asked: 2020-12-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of SMPD1 causing degradation of its protein structure. SMPD1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SMPD1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-23
Question
Q: if the enzyme conjugated SMPD1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SMPD1 antigen?
Verified Customer
Verified customer
Asked: 2020-11-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SMPD1 using the EK2025 Human SMPD1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
C. Moore
Verified customer
Asked: 2018-12-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-12-27
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-22
