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Product Info Summary
| SKU: | PROTP01574-4 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant IFN beta 1a (Interferon beta 1a) protein, AF
View all IFN-beta recombinant proteins
SKU/Catalog Number
PROTP01574-4
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Interferon-Beta 1a (IFN-beta 1a) is a leukocyte interferon, which is a variant of Interferon-beta. IFN-beta 1a is a 19 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant IFN beta 1a (Interferon beta 1a) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01574-4)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
22.294kDa
Molecular weight
The protein has a calculated MW of 20.84 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce apoptosis in HeLa cells. The ED₅₀ for this effect is <15 ng/mL. Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human IFN beta 1a is approximately >1 x10⁷ IU/ mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human IFN beta 1a
Protein Target Info & Infographic
Gene/Protein Information For IFNB1 (Source: Uniprot.org, NCBI)
Gene Name
IFNB1
Full Name
Interferon beta
Weight
22.294kDa
Superfamily
alpha/beta interferon family
Alternative Names
IFNB1, Type I Interferon IFNB1 IFB, IFF, IFN-beta, IFNB interferon beta 1 interferon beta|fibroblast interferon|interferon, beta 1, fibroblast|interferon-beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IFNB1, check out the IFNB1 Infographic
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In this infographic, you will see the following information for IFNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant IFN beta 1a (Interferon beta 1a) protein, AF (PROTP01574-4)
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