Human recombinant FGF-6 (Fibroblast growth factor-6) protein, AF

FGF-6 protein, Human

Fibroblast Growth Factors-6 (FGF-6) is a 22.9 kDa member of the fibroblast Growth Factors with 208 amino acid residues. FGF-6 is an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis. In physiological function, FGF6 is required for normal muscle regeneration.

Product Info Summary

SKU: PROTP10767-2
Size: 5ug,20ug,100ug
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant FGF-6 (Fibroblast growth factor-6) protein, AF

View all FGF-6 recombinant proteins

SKU/Catalog Number

PROTP10767-2

Size

5ug,20ug,100ug

Tag

His Tag (C-term)

Description

Fibroblast Growth Factors-6 (FGF-6) is a 22.9 kDa member of the fibroblast Growth Factors with 208 amino acid residues. FGF-6 is an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis. In physiological function, FGF6 is required for normal muscle regeneration.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant FGF-6 (Fibroblast growth factor-6) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP10767-2)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

22.905kDa

Molecular weight

The protein has a calculated MW of 19.66 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human FGF-6 is > 1 x 10⁷ IU/mg.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

MGTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI with polyhistidine tag at the C-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For FGF6 (Source: Uniprot.org, NCBI)

Gene Name

FGF6

Full Name

Fibroblast growth factor 6

Weight

22.905kDa

Superfamily

heparin-binding growth factors family

Alternative Names

HBGF-6, HST-2 FGF6 HBGF-6, HST2 fibroblast growth factor 6 fibroblast growth factor 6|FGF-6|HST-2|HSTF-2|heparin secretory-transforming protein 2|heparin-binding growth factor 6

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on FGF6, check out the FGF6 Infographic

FGF6 infographic

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In this infographic, you will see the following information for FGF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP10767-2

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