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Product Info Summary
| SKU: | PROTP08620-3 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant FGF-4 (Fibroblast growth factor-4) protein, AF
View all FGF-4 recombinant proteins
SKU/Catalog Number
PROTP08620-3
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Fibroblast Growth Factors-4 (FGF-4) is a 22 kDa member of the fibroblast Growth Factors with 206 amino acid residues. FGF-4 can regulate embryonic development, cell proliferation, and cell differentiation. FGF-4 is an important role development during embryogenesis.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant FGF-4 (Fibroblast growth factor-4) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP08620-3)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 0.1% sarkosyl in 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
22.048kDa
Molecular weight
The protein has a calculated MW of 20.70 kDa. The protein migrates as 22 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2.5 ng/mL. The specific activity of recombinant human FGF-4 is > 4 x 10⁵ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MGRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL with polyhistidine tag at the C-terminus
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human FGF-4
Protein Target Info & Infographic
Gene/Protein Information For FGF4 (Source: Uniprot.org, NCBI)
Gene Name
FGF4
Full Name
Fibroblast growth factor 4
Weight
22.048kDa
Superfamily
heparin-binding growth factors family
Alternative Names
HST-1, Transforming protein KS3, HBGF-4 FGF4 FGF-4, HBGF-4, HST, HST-1, HSTF-1, HSTF1, K-FGF, KFGF fibroblast growth factor 4 fibroblast growth factor 4|heparin secretory transforming protein 1|heparin-binding growth factor 4|human stomach cancer, transforming factor from FGF-related oncogene|kaposi sarcoma oncogene|oncogene HST|transforming protein KS3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FGF4, check out the FGF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant FGF-4 (Fibroblast growth factor-4) protein, AF (PROTP08620-3)
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