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Product Info Summary
| SKU: | PROTQ6KF10-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant BMP-13 (Bone morphogenetic protein-13) protein, AF
View all GDF-6/BMP-13 recombinant proteins
SKU/Catalog Number
PROTQ6KF10-2
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Bone Morphogenetic Protein-13 (BMP-13), known as Growth differentiation factor 6 (GDF6), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-13 can bind with the TGFβ receptor and induce SMAD protein signal transduction. BMP-13 is a regulatory protein that can affect the growth and differentiation of developing embryos. Moreover, it plays a role in regulating the patterning of the ectoderm and controlling eye development.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant BMP-13 (Bone morphogenetic protein-13) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ6KF10-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
50.942kDa
Molecular weight
The protein has a calculated MW of 14.50 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 63-240 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MTAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human BMP-13
Protein Target Info & Infographic
Gene/Protein Information For GDF6 (Source: Uniprot.org, NCBI)
Gene Name
GDF6
Full Name
Growth/differentiation factor 6
Weight
50.942kDa
Superfamily
TGF-beta family
Alternative Names
subunit (CLMF p35), NK cell Stimulating Factor Chain 1 Gdf6|BMP-13, BMP1, BMP13, GDF16|growth differentiation factor 6|growth/differentiation factor 6|GDF-6|bone morphogenetic protein 13|growth/differentiation factor 16
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on GDF6, check out the GDF6 Infographic
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In this infographic, you will see the following information for GDF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant BMP-13 (Bone morphogenetic protein-13) protein, AF (PROTQ6KF10-2)
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