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Product Info Summary
| SKU: | EK2265 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PDGFRA ELISA Kit PicoKine®
View all PDGFR alpha ELISA kits
SKU/Catalog Number
EK2265
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PDGFRA ELISA Kit PicoKine® (96 Tests). Quantitate Human PDGFRA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PDGFRA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2265)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q24-E524
Sensitivity
<45 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2265 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2265 is reactive to PDGFRA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2265 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDGFR alpha
Platelet-derived growth factor receptor A, also termed CD140a, is a receptor located on the surface of a wide range of cell types. The protein is encoded in the human by the PDGFRA gene. This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2265-CAP | Anti-Human PDGFRA Pre-coated 96-well strip microplate | 1 |
| EK2265-ST | Human PDGFRA Standard | 2 vials, 10 ng/tube |
| EK2265-DA | Human PDGFRA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.029 | 0.099 | 0.186 | 0.397 | 0.863 | 1.273 | 1.579 | 2.097 |
Data Example Images
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Human PDGFRA PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PDGFRA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 127 | 317 | 2663 | 109 | 345 | 2513 |
| Standard deviation | 2.63 | 16.52 | 154.72 | 3.71 | 23.87 | 79.66 |
| CV(%) | 2.1% | 5.2% | 5.8% | 3.4% | 6.9% | 3.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PDGFR alpha in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 127 | 183 | 95 | 142 | 137 | 4.39 | 3.2% |
| Sample 2 | 317 | 299 | 328 | 202 | 287 | 9.43 | 3.3% |
| Sample 3 | 2663 | 1607 | 1919 | 1566 | 1939 | 132.42 | 6.8% |
Protein Target Info & Infographic
Gene/Protein Information For PDGFRA (Source: Uniprot.Org, NCBI)
Gene Name
PDGFRA
Full Name
Platelet-derived growth factor receptor alpha
Weight
122.67kDa
Superfamily
protein kinase superfamily
Alternative Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA PDGFRA CD140A, PDGFR-2, PDGFR2 platelet derived growth factor receptor alpha platelet-derived growth factor receptor alpha|CD140 -like family member A|CD140a |PDGF-R-alpha|alpha-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 2|platelet-derived growth factor receptor, alpha polypeptide
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PDGFRA, check out the PDGFRA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDGFRA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PDGFRA ELISA Kit PicoKine® (EK2265)
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