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Product Info Summary
| SKU: | EK2273 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Nidogen2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2273
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Nidogen2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Nidogen2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Nidogen2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2273)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: L31-K1375
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2273 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2273 is reactive to NID2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2273 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Nidogen-2
This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2273-CAP | Anti-Human NID2 Pre-coated 96-well strip microplate | 1 |
| EK2273-ST | Human NID2 Standard | 2 vials, 10 ng/tube |
| EK2273-DA | Human NID2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.042 | 0.167 | 0.234 | 0.412 | 0.663 | 0.935 | 1.345 | 2.078 |
Data Example Images
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Human Nidogen2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Nidogen2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 217 | 475 | 3201 | 191 | 346 | 3527 |
| Standard deviation | 16.14 | 24.61 | 114.28 | 11.56 | 14.81 | 232.78 |
| CV(%) | 7.4% | 5.2% | 3.6% | 6.1% | 4.3% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Nidogen-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 217 | 187 | 287 | 163 | 214 | 5.64 | 2.6% |
| Sample 2 | 475 | 399 | 511 | 593 | 495 | 37.19 | 7.5% |
| Sample 3 | 3201 | 4223 | 3220 | 4079 | 3681 | 237.04 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For NID2 (Source: Uniprot.Org, NCBI)
Gene Name
NID2
Full Name
Nidogen-2
Weight
153.913kDa
Alternative Names
NID2; NID-2; nidogen 2 (osteonidogen); Nidogen2; Nidogen-2; Osteonidogen Nid2|AW547149, Ly111, NID-2, entact, entactin-2, nidogen-2|nidogen 2|nidogen-2|entactin 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NID2, check out the NID2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NID2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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