Human IL-1β PicoKine® Quick ELISA Kit

IL-1 beta/IL-1F2 ELISA kit, 90-minute one-step protocol

Human IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: FEK0392
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay Time: 90 Minutes

Product Name

Human IL-1β PicoKine® Quick ELISA Kit

See all IL-1 beta/IL-1F2 products

SKU/Catalog Number

FEK0392

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human IL-1β PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0392)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: A117-S269

Sensitivity

<0.15 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of FEK0392 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0392 is reactive to IL1B in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

FEK0392 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-1 beta/IL-1F2

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Kit Components

Catalog numberDescription QuantityVolume
FEK0392-CAPAnti-Human IL-1 beta/IL-1F2 Pre-coated 96-well Strip Microplate 112 strips of 8 wells
-STHuman IL-1 beta/IL-1F2 Standard 2 1 ng/tube
FEK0392-HHRP Linked Anti-Human IL1B Antibody 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0220.0740.1050.2490.4230.7071.0951.830

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-1β PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11601231058112
Standard deviation0.452.888.970.573.249.96
CV (%)4.1%4.8%7.3%5.7%5.6%8.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 111101111100.434.3%
Sample 260545253543.115.7%
Sample 31231191231121194.493.7%
*number of samples for each test n=16.

Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)

Gene Name

IL1B

Full Name

Interleukin-1 beta

Weight

30.748kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1B, check out the IL1B Infographic

IL1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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