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Product Info Summary
| SKU: | FEK0373 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0373
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human IFNG in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0373)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q24-Q166
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0373 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0373 is reactive to IFNG in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
FEK0373 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFN-gamma
Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0373-CAP | Anti-Human IFN-gamma Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0373-ST | Human IFN-gamma Standard | 2 | 10 ng/tube |
| FEK0373-H | HRP Linked Anti-Human IFNG Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.023 | 0.098 | 0.167 | 0.314 | 0.595 | 0.748 | 1.846 | 2.068 |
Data Example Images
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Human IFN Gamma PicoKine Quick ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 38 | 37 | 36 | 36 | 1.47 | 4% |
| Sample 2 | 150 | 152 | 164 | 166 | 158 | 7.07 | 4.4% |
| Sample 3 | 469 | 446 | 458 | 468 | 460 | 9.28 | 2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 150 | 469 | 33 | 139 | 437 |
| Standard deviation | 1.73 | 8.7 | 29.54 | 1.91 | 9.73 | 31.9 |
| CV (%) | 7.3% | 5.8% | 7% | 5.8% | 7% | 7.3% |
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.Org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19.348kDa
Superfamily
type II (or gamma) interferon family
Alternative Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit (FEK0373)
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