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Product Info Summary
| SKU: | EK2286 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IFN-Beta ELISA Kit PicoKine®
SKU/Catalog Number
EK2286
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IFN-Beta ELISA Kit PicoKine®(96 Tests). Quantitate Human IFNB1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml.
Storage & Handling
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IFN-Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2286)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: M22-N187
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2286 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2286 is reactive to IFNB1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2286 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFN-beta
Interferon beta is a protein that in humans is encoded by the IFNB1 gene. This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2286-CAP | Anti-Human IFNB1 Pre-coated 96-well strip microplate | 1 |
| EK2286-ST | Human IFNB1 Standard | 2 vials, 10 ng/tube |
| EK2286-DA | Human IFNB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.018 | 0.066 | 0.186 | 0.211 | 0.471 | 0.824 | 1.552 | 1.999 |
Data Example Images
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Human IFN-Beta ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IFN-Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 27 | 124 | 428 | 28 | 97 | 299 |
| Standard deviation | 1.2 | 4.18 | 22.6 | 2.13 | 6.56 | 11.48 |
| CV(%) | 4.5% | 3.4% | 5.3% | 7.6% | 6.8% | 3.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IFN-beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 18 | 27 | 27 | 25 | 0.67 | 2.7% |
| Sample 2 | 124 | 75 | 65 | 112 | 94 | 6.5 | 6.9% |
| Sample 3 | 428 | 383 | 386 | 368 | 391 | 11.46 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For IFNB1 (Source: Uniprot.Org, NCBI)
Gene Name
IFNB1
Full Name
Interferon beta
Weight
22 kDa
Superfamily
alpha/beta interferon family
Alternative Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956 IFNB1 IFB, IFF, IFN-beta, IFNB interferon beta 1 interferon beta|fibroblast interferon|interferon, beta 1, fibroblast|interferon-beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNB1, check out the IFNB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IFN-Beta ELISA Kit PicoKine® (EK2286)
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