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Product Info Summary
| SKU: | EK2214 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human GUSB ELISA Kit PicoKine®
View all beta-Glucuronidase/GUSB ELISA kits
SKU/Catalog Number
EK2214
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GUSB Kit PicoKine® (96 Tests). Quantitate Human GUSB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GUSB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2214)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M1-T651
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2214 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2214 is reactive to GUSB in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2214 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of beta-Glucuronidase/GUSB
This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2214-CAP | Anti-Human GUSB Pre-coated 96-well strip microplate | 1 |
| EK2214-ST | Human GUSB Standard | 2 vials, 10 ng/tube |
| EK2214-DA | Human GUSB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.022 | 0.053 | 0.151 | 0.217 | 0.433 | 0.878 | 1.394 | 1.992 |
Data Example Images
Click image to see more details
Human GUSB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GUSB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 362 | 1379 | 4926 | 388 | 1295 | 4782 |
| Standard deviation | 19.91 | 70.33 | 241.37 | 16.68 | 62.16 | 186.5 |
| CV(%) | 5.5% | 5.1% | 4.9% | 4.3% | 4.8% | 3.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of beta-Glucuronidase/GUSB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 362 | 363 | 367 | 372 | 366 | 15.74 | 4.3% |
| Sample 2 | 1379 | 1368 | 1385 | 1296 | 1357 | 74.64 | 5.5% |
| Sample 3 | 4926 | 4593 | 4768 | 4852 | 4785 | 282.30 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For GUSB (Source: Uniprot.Org, NCBI)
Gene Name
GUSB
Full Name
Beta-glucuronidase
Weight
74.732kDa
Superfamily
glycosyl hydrolase 2 family
Alternative Names
beta-D-glucuronidase; Beta-G1; beta-glucuronidase; BG; EC 3.2.1.31; FLJ39445; glucuronidase, beta; GUSB; MPS7 GUSB BG, MPS7 glucuronidase beta beta-glucuronidase|beta-D-glucuronidase|beta-G1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GUSB, check out the GUSB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GUSB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GUSB ELISA Kit PicoKine® (EK2214)
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