Product Info Summary
SKU: | EK7055 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human Ferritin OneStep ELISA Kit
SKU/Catalog Number
EK7055
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ferritin (Ferr). 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Ferritin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7055)
Clonality of Antibodies
See Datasheet for details
Sensitivity
2.5 ng/mL
Assay Range
5-160 ng/mL
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7055 is reactive to Fth1 in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7055 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Anti-Human Ferritin (Ferr) Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- Ferritin (Ferr) monoclonal antibody, Polystyrene micro-well plate |
Human Ferritin (Ferr) Standards (S0~S5) | 6 | 0.5ml | Ferritin (Ferr) (0, 5, 15, 35, 80, 160 ng/mL), 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300 |
HRP Conjugated anti-Human Ferritin (Ferr) antibody | 1 | 6ml | HRP Conjugated anti-Human Ferritin (Ferr) antibody, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300, 0.01% azophloxine |
Controls | 2 | 0.5ml | Ferritin (Ferr), 100% natural protein, 0.1% Proclin-300 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Ferritin(Ferr) ELISA Kit standard curve
Specific Publications For Human Ferritin OneStep ELISA Kit (EK7055)
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