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Product Info Summary
| SKU: | EK0945 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Endothelin ELISA Kit PicoKine®
SKU/Catalog Number
EK0945
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Endothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human Endothelin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Endothelin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0945)
Clonality of Antibodies
See Datasheet for details
Standard Protein
A peptide reflecting the target protein in human. Sequence: C53-W73
Sensitivity
<0.5 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
See datasheet of EK0945 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0945 is reactive to Endothelin in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0945 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endothelin
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0945-CAP | Anti-Human Endothelin Pre-coated 96-well strip microplate | 1 |
| EK0945-ST | Human Endothelin Standard | 2 vials, 1 ng/tube |
| EK0945-DA | Human Endothelin Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.001 | 0.039 | 0.064 | 0.149 | 0.301 | 0.680 | 1.150 | 1.821 |
Data Example Images
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Human Endothelin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Endothelin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 11 | 51 | 131 | 11 | 51 | 130 |
| Standard deviation | 0.62 | 2.75 | 8.64 | 0.62 | 2.95 | 10.92 |
| CV (%) | 5.7% | 5.4% | 6.6% | 5.7% | 5.8% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Endothelin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 11 | 13 | 12 | 14 | 12 | 1.11 | 9.2% |
| Sample 2 | 51 | 49 | 47 | 47 | 48 | 1.65 | 3.4% |
| Sample 3 | 131 | 117 | 110 | 124 | 120 | 7.82 | 6.5% |
Specific Publications For Human Endothelin ELISA Kit PicoKine® (EK0945)
Hello CJ!
EK0945 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng Hj, Dai Dz, Ji H, Dai Y. Eur J Pharmacol. 2010 May 25;634(1-3):101-6. Doi: 10.1016/J.Ejphar.2010.02.001. Epub 2010 Feb 16. The Separate Roles Of Endothelin Receptors Participate In Remodeling Of Matrix Metalloproteinase And Connexin 43 Of Car...
Liu B, Zhu Y, Zhou J, Wei Y, Long C, Chen M, Ling Y, Ge J, Zhuo Y. Cell Stress Chaperones. 2014 Nov;19(6):827-35. Doi: 10.1007/S12192-014-0506-7. Epub 2014 Mar 19. Endoplasmic Reticulum Stress Promotes Amyloid-Beta Peptides Production In Rgc-5 Cells.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human Endothelin ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Z. Mangal
Verified customer
Asked: 2019-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-22
Question
Does EK0945 contain the Human Serum Protein as standard protein?
Verified customer
Asked: 2019-05-20
Answer
For the Human Endothelin 1 ELISA Kit PicoKine™ (EK0945), the standard is a human peptide.
Boster Scientific Support
Answered: 2019-05-22
Question
Q: is it okay to use citrate plasma as samples in Human Endothelin-1 Picokine® ELISA Kit (Catalog # EK0945)?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Endothelin-1 causing disruption of its protein structure. Endothelin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Endothelin-1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-07-25
Question
Q: Can Endothelin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-19
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-01-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Endothelin-1 using the EK0945 Human Endothelin-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-10-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-02-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-02-10
Question
Q: if the enzyme conjugated Endothelin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endothelin-1 antigen?
Verified Customer
Verified customer
Asked: 2016-01-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-01-19
