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Product Info Summary
| SKU: | EK2188 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human DSCAML1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2188
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DSCAML1 ELISA Kit PicoKine® (96 Tests). Quantitate Human DSCAML1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DSCAML1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2188)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E79-K1651
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2188 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2188 is reactive to DSCAML1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2188 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DSCAM-L1
Down syndrome cell adhesion molecule like 1 is a protein in humans that is encoded by the DSCAML1 gene. The protein encoded by this gene is a member of the Ig superfamily of cell adhesion molecules and is involved in neuronal differentiation. The encoded membrane-bound protein localizes to the cell surface, where it forms aggregates that repel neuronal processes of the same cell type.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2188-CAP | Anti-Human DSCAML1 Pre-coated 96-well strip microplate | 1 |
| EK2188-ST | Human DSCAML1 Standard | 2 vials, 10 ng/tube |
| EK2188-DA | Human DSCAML1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.032 | 0.108 | 0.183 | 0.341 | 0.562 | 0.924 | 1.453 | 2.014 |
Data Example Images
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Human DSCAML1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DSCAML1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 268 | 1355 | 5652 | 271 | 1403 | 5594 |
| Standard deviation | 6.16 | 48.78 | 180.86 | 11.11 | 58.93 | 246.14 |
| CV (%) | 2.3% | 3.6% | 3.2% | 4.1% | 4.2 | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DSCAM-L1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 268 | 196 | 228 | 231 | 231 | 12 | 5.2% |
| Sample 2 | 1355 | 1403 | 1365 | 1396 | 1380 | 77.27 | 5.6% |
| Sample 3 | 5652 | 5568 | 5463 | 5399 | 5521 | 264.98 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For DSCAML1 (Source: Uniprot.Org, NCBI)
Gene Name
DSCAML1
Full Name
Down syndrome cell adhesion molecule-like protein 1
Weight
224.463kDa
Alternative Names
Down syndrome cell adhesion molecule like 1; Down syndrome cell adhesion molecule-like protein 1; Downs syndrome cell adhesion molecule like 1; DSCAM2; DSCAML1; DSCAM-L1; KIAA1132Down syndrome cell adhesion molecule 2 DSCAML1 DSCAM2 DS cell adhesion molecule like 1 Down syndrome cell adhesion molecule-like protein 1|DSCAM-like 1|Downs syndrome cell adhesion molecule like 1|down syndrome cell adhesion molecule 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DSCAML1, check out the DSCAML1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DSCAML1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DSCAML1 ELISA Kit PicoKine® (EK2188)
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