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Product Info Summary
| SKU: | EK2186 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human DPP7 ELISA Kit PicoKine®
View all DPPII/QPP/DPP7 ELISA kits
SKU/Catalog Number
EK2186
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DPP7 ELISA Kit PicoKine® (96 Tests). Quantitate Human DPP7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DPP7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2186)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G22-L492
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2186 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2186 is reactive to DPP7 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2186 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DPPII/QPP/DPP7
Dipeptidyl-peptidase 2 is an enzyme that in humans is encoded by the DPP7 gene. The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2186-CAP | Anti-Human DPP7 Pre-coated 96-well strip microplate | 1 |
| EK2186-ST | Human DPP7 Standard | 2 vials, 10 ng/tube |
| EK2186-DA | Human DPP7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.068 | 0.156 | 0.235 | 0.348 | 0.696 | 1.112 | 1.785 | 2.395 |
Data Example Images
Click image to see more details
Human DPP7 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DPP7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 86 | 212 | 922 | 95 | 226 | 892 |
| Standard deviation | 4.39 | 11.87 | 56.24 | 3.71 | 10.85 | 37.46 |
| CV (%) | 5.1% | 5.6% | 6.1% | 3.9% | 4.8% | 4.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DPPII/QPP/DPP7 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 86 | 81 | 85 | 89 | 85 | 2.73 | 3.2% |
| Sample 2 | 212 | 226 | 208 | 215 | 215 | 9.47 | 4.4% |
| Sample 3 | 922 | 965 | 942 | 951 | 945 | 38.75 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For DPP7 (Source: Uniprot.Org, NCBI)
Gene Name
DPP7
Full Name
Dipeptidyl peptidase 2
Weight
54.341kDa
Superfamily
peptidase S28 family
Alternative Names
carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase DPP7 DPP2, DPPII, QPP dipeptidyl peptidase 7 dipeptidyl peptidase 2|DPP II|carboxytripeptidase|dipeptidyl aminopeptidase II|dipeptidyl arylamidase II|dipeptidyl-peptidase II|quiescent cell proline dipeptidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DPP7, check out the DPP7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DPP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DPP7 ELISA Kit PicoKine® (EK2186)
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