Human CXCL16 PicoKine® Quick ELISA Kit

Product Name

Human CXCL16 PicoKine® Quick ELISA Kit

See all CXCL16 products

SKU/Catalog Number

FEK0741

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL16 PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human CXCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CXCL16 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0741)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: N49-P137

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0741 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0741 is reactive to CXCL16 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0741 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CXCL16

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0741-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0741-ST	Human CXCL16 Standard	2	10 ng/tube
FEK0741-A	Human CXCL16 Antibody A	1	3ml
FEK0741-B	Human CXCL16 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.096	0.164	0.291	0.433	0.715	1.073	1.593	2.046

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CXCL16 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL16 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CXCL16 (Source: Uniprot.Org, NCBI)

Alternative Names

chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16 CXCL16 CXCLG16, SR-PSOX, SRPSOX C-X-C motif chemokine ligand 16 C-X-C motif chemokine 16|CXC chemokine ligand 16|chemokine (C-X-C motif) ligand 16|scavenger receptor for phosphatidylserine and oxidized low density lipoprotein|small-inducible cytokine B16|transmembrane chemokine CXCL16

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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