Human CSPG5 ELISA Kit PicoKine®

Product Name

Human CSPG5 ELISA Kit PicoKine®

View all Neuroglycan C/CSPG5 ELISA kits

SKU/Catalog Number

EK2173

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CSPG5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CSPG5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CSPG5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2173)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO; Immunogen sequence: V31-Q420

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK2173 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2173 is reactive to CSPG5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2173 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Neuroglycan C/CSPG5

Chondroitin sulfate proteoglycan 5 is a protein that in humans is encoded by the CSPG5 gene. The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene.

Kit Components

Catalog Number	Description	Quantity
EK2173-CAP	Anti-Human CSPG5 Pre-coated 96-well strip microplate	1
EK2173-ST	Human CSPG5 Standard	2 vials, 10 ng/tube
EK2173-DA	Human CSPG5 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.032	0.065	0.131	0.274	0.559	1.178	1.982	2.141

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CSPG5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Neuroglycan C/CSPG5 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CSPG5 (Source: Uniprot.Org, NCBI)

Alternative Names

CALEB; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; CSPG5; MGC44034; Neuroglycan C; NGC; NGCAcidic leucine-rich EGF-like domain-containing brain protein CSPG5 NGC chondroitin sulfate proteoglycan 5 chondroitin sulfate proteoglycan 5|acidic leucine-rich EGF-like domain-containing brain protein|chondroitin sulfate proteoglycan 5 (neuroglycan C)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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