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Product Info Summary
| SKU: | FEK1253 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit
See all CLEC3B/Tetranectin products
SKU/Catalog Number
FEK1253
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CLEC3B in cell culture supernatants and serum. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1253)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E22-V202
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1253 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1253 is reactive to CLEC3B in Human samples
Validated Sample Types
cell culture supernatants and serum
Application Guarantee
FEK1253 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CLEC3B/Tetranectin
Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1253-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1253-ST | Human CLEC3B/Tetranectin Standard | 2 | 20 ng/tube |
| FEK1253-A | Human CLEC3B Antibody A | 1 | 3ml |
| FEK1253-B | Human CLEC3B Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.002 | 0.123 | 0.215 | 0.377 | 0.703 | 1.153 | 1.707 | 2.189 |
Data Example Images
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Human CLEC3B PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 432 | 2688 | 8213 | 410 | 2552 | 7809 |
| Standard deviation | 26.35 | 118.27 | 459.92 | 31.16 | 137.80 | 554.43 |
| CV(%) | 6.1% | 4.4% | 5.6% | 7.6% | 5.4% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CLEC3B/Tetranectin in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 432 | 486 | 505 | 451 | 468 | 29.01 | 6.2% |
| Sample 2 | 2688 | 2756 | 2929 | 3088 | 2865 | 163.30 | 5.7% |
| Sample 3 | 8213 | 9092 | 8519 | 7750 | 8393 | 436.43 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For CLEC3B (Source: Uniprot.Org, NCBI)
Gene Name
CLEC3B
Full Name
Tetranectin
Weight
22.537kDa
Alternative Names
CLEC3B; C-type lectin domain family 3 member B; C-type lectin domain family 3, member B; DKFZp686H17246; Plasminogen kringle 4-binding protein; TETN; tetranectin (plasminogen binding protein); Tetranectin; TNA; TNAtetranectin (plasminogen-binding protein); TNtetranectin CLEC3B TN, TNA C-type lectin domain family 3 member B tetranectin|plasminogen kringle 4-binding protein|tetranectin (plasminogen-binding protein)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CLEC3B, check out the CLEC3B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLEC3B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit (FEK1253)
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