Human CES1 PicoKine® Quick ELISA Kit

Product Name

Human CES1 PicoKine® Quick ELISA Kit

See all Carboxylesterase 1/CES1 products

SKU/Catalog Number

FEK1813

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CES1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CES1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1 ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CES1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1813)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: G18-L567

Sensitivity

<0.1 ng/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

See datasheet of FEK1813 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1813 is reactive to CES1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

FEK1813 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Carboxylesterase 1/CES1

Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Kit Components

Catalog number	Description	Quantity	Volume
FEK1813-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK1813-ST	Human Carboxylesterase 1/CES1 Standard	2	50 ng/tube
FEK1813-A	Human CES1 Antibody A	1	3ml
FEK1813-B	Human CES1 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.005	0.093	0.154	0.281	0.561	0.934	1.358	2.129

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CES1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carboxylesterase 1/CES1 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CES1 (Source: Uniprot.Org, NCBI)

Alternative Names

ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; carboxylesterase 2 (liver); CEH; CES1; Ces-1; CES1A2; CES2EC 3.1.1.1; cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1; Egasyn; HMSE1; HMSECES1A1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; MGC117365; Monocyte/macrophage serine esterase; PCE-1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; Ses-1; SES1TGH; TGH; Triacylglycerol hydrolase CES1 ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1 carboxylesterase 1 liver carboxylesterase 1|acyl coenzyme A:cholesterol acyltransferase|brain carboxylesterase hBr1|carboxylesterase 1 (monocyte/macrophage serine esterase 1)|carboxylesterase 2 (liver)|cholesteryl ester hydrolase|cocaine carboxylesterase|egasyn|human monocyte/macrophage serine esterase 1|methylumbelliferyl-acetate deacetylase 1|monocyte/macrophage serine esterase|retinyl ester hydrolase|serine esterase 1|triacylglycerol hydrolase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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