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Product Info Summary
| SKU: | EK2200 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD89 ELISA Kit PicoKine®
SKU/Catalog Number
EK2200
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD89 Kit PicoKine® (96 Tests). Quantitate Human FCAR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD89 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2200)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q22-N227
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2200 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2200 is reactive to FCAR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2200 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCAR/CD89
This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2200-CAP | Anti-Human FCAR Pre-coated 96-well strip microplate | 1 |
| EK2200-ST | Human FCAR Standard | 2 vials, 10 ng/tube |
| EK2200-DA | Human FCAR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.045 | 0.118 | 0.189 | 0.296 | 0.484 | 0.754 | 1.496 | 2.071 |
Data Example Images
Click image to see more details
Human CD89 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD89 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 23 | 67 | 267 | 25 | 70 | 275 |
| Standard deviation | 1.29 | 3.89 | 16.55 | 1.73 | 4.97 | 17.88 |
| CV (%) | 5.6% | 5.8% | 6.2% | 6.9% | 7.1% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCAR/CD89 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 23 | 25 | 29 | 27 | 26 | 1.69 | 6.5% |
| Sample 2 | 67 | 68 | 75 | 88 | 75 | 3.13 | 4.2% |
| Sample 3 | 267 | 296 | 270 | 311 | 286 | 13.16 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For FCAR (Source: Uniprot.Org, NCBI)
Gene Name
FCAR
Full Name
Immunoglobulin alpha Fc receptor
Weight
32.265kDa
Alternative Names
CD89 antigen; CD89; CD89IgA Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; FCAR; immunoglobulin alpha Fc receptor FCAR CD89, CTB-61M7.2, FcalphaRI Fc fragment of IgA receptor immunoglobulin alpha Fc receptor|FCAR variant 14|Fc alpha receptor|Fc fragment of IgA, receptor for
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCAR, check out the FCAR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCAR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD89 ELISA Kit PicoKine® (EK2200)
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