Human CD49A ELISA Kit PicoKine®

Integrin alpha 1/CD49a ELISA kit for Human

Human CD49A PicoKine® ELISA Kit (96 Tests). Quantitate Human CD49A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2225
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CD49A ELISA Kit PicoKine®

View all Integrin alpha 1/CD49a ELISA kits

SKU/Catalog Number

EK2225

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD49A PicoKine® ELISA Kit (96 Tests). Quantitate Human CD49A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD49A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2225)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO; Immunogen sequence: M1-P1141

Sensitivity

<75 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2225 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2225 is reactive to ITGA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2225 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Integrin alpha 1/CD49a

Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.

Kit Components

Catalog Number Description Quantity
EK2225-CAP Anti-Human ITGA1 Pre-coated 96-well strip microplate 1
EK2225-ST Human ITGA1 Standard 2 vials, 10 ng/tube
EK2225-DA Human ITGA1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0420.0870.1630.3950.8141.2411.5251.842

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD49A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16159239072026174838
Standard deviation9.7712.31100.027.8435.79166.91
CV(%)6.1%2.1%2.6%3.9%5.8%3.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Integrin alpha 1/CD49a in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11612681731962005.832.9%
Sample 259262536834548319.354%
Sample 339073743315045193830288.767.5%
*number of samples for each test n=16.

Gene/Protein Information For ITGA1 (Source: Uniprot.Org, NCBI)

Gene Name

ITGA1

Full Name

Integrin alpha-1

Weight

130.848kDa

Superfamily

integrin alpha chain family

Alternative Names

CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1 ITGA1 CD49a, VLA1 integrin subunit alpha 1 integrin alpha-1|CD49 -like family member A|VLA-1|laminin and collagen receptor|very late activation protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ITGA1, check out the ITGA1 Infographic

ITGA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ITGA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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